Tranmere Rovers v Southampton, 20 February 2001
| Score | 4-3 to Tranmere Rovers |
| Referee | Steve Dunn |
| Competition | FA Cup 5th round replay |
| Venue | Prenton Park |
| Attendance | 12,910 |
View Tranmere Rovers v Southampton head to head
Tranmere produce one of the greatest comebacks in FA Cup history, 3-0 down at half-time and seemingly out of the Cup, with the help of a Paul Rideout hat-trick in 21 minutes and a goal 7 minutes from time by substitute Stuart Barlow they Southampton to their list of Premiership scalps. John Aldridge's boys now have a home tie against their Merseyside neighbours Liverpool
Goals:
| Paul Rideout | 59 |
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| Paul Rideout | 71 |
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| Paul Rideout | 80 |
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| Stuart Barlow | 83 |
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Starting lineup:
Goalkeeper
John Achterberg
Defender
Graham Allen
Defender
Richard Jobson
Defender
Steve Yates
Defender/Centre back
Dave Challinor
Defender/Midfielder
Richard Hinds
Midfielder
Sean Flynn
Midfielder
Des Hamilton
Midfielder
Jason Koumas
Forward
Andy Parkinson
Forward
Paul Rideout
Substitutions:
| Nick Henry for Des Hamilton | 46 |
| Stuart Barlow for Dave Challinor | 46 |
| Gareth Roberts for Jason Koumas | 89 |
Cards:
| Stuart Barlow | 81 | Y |
Goals:
| Hassan Kachloul | 12 |
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| Jo Tessem | 26 |
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| Dean Richards | 45 |
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Starting lineup:
Paul Jones
Goalkeeper
Wayne Bridge
Defender
Claus Lundekvam
Defender
Dean Richards
Defender/Centre back
Mark Draper
Midfielder
Kevin Gibbens
Midfielder
Hassan Kachloul
Midfielder
Chris Marsden
Midfielder
Jo Tessem
Midfielder
James Beattie
Forward
Marian Pahars
Forward
Substitutions:
| Imants Bleidelis for Marian Pahars | 46 |
| Uwe Rosler for Imants Bleidelis | 85 |
Cards:
| James Beattie | 22 | Y |
| Chris Marsden | 80 | Y |