The table below shows Gainsborough Trinity's overall record against each of their former football league opponents.
| Opposition | P | W | D | L | F | A |
|---|---|---|---|---|---|---|
| Accrington Stanley | 1 | 0 | 0 | 1 | 1 | 2 |
| Aldershot | 1 | 0 | 0 | 1 | 0 | 2 |
| Altrincham | 1 | 1 | 0 | 0 | 1 | 0 |
| Barnet | 1 | 0 | 0 | 1 | 1 | 3 |
| Barnsley | 28 | 10 | 6 | 12 | 39 | 48 |
| Birmingham City | 8 | 2 | 4 | 2 | 8 | 13 |
| Blackpool | 31 | 12 | 9 | 10 | 42 | 40 |
| Blyth Spartans | 1 | 1 | 0 | 0 | 3 | 1 |
| Bolton Wanderers | 10 | 3 | 1 | 6 | 8 | 27 |
| Boston Town | 1 | 1 | 0 | 0 | 7 | 0 |
| Bradford City | 10 | 5 | 1 | 4 | 21 | 24 |
| Bradford Park Avenue | 8 | 2 | 1 | 5 | 7 | 20 |
| Brentford | 2 | 0 | 0 | 2 | 1 | 9 |
| Bristol City | 12 | 4 | 1 | 7 | 16 | 21 |
| Burnley | 27 | 8 | 4 | 15 | 30 | 42 |
| Burslem Port Vale | 3 | 2 | 0 | 1 | 7 | 4 |
| Burton Swifts | 10 | 4 | 2 | 4 | 17 | 16 |
| Burton United | 12 | 5 | 1 | 6 | 19 | 23 |
| Burton Wanderers | 2 | 1 | 0 | 1 | 4 | 4 |
| Chelsea | 8 | 2 | 1 | 5 | 8 | 15 |
| Chesterfield | 2 | 0 | 0 | 2 | 3 | 7 |
| Chesterfield Town | 20 | 7 | 5 | 8 | 30 | 39 |
| Colchester United | 1 | 0 | 0 | 1 | 0 | 1 |
| Crewe Alexandra | 3 | 2 | 1 | 0 | 6 | 3 |
| Darlington | 1 | 0 | 0 | 1 | 1 | 2 |
| Dartford | 1 | 0 | 0 | 1 | 0 | 4 |
| Darwen | 6 | 3 | 1 | 2 | 16 | 12 |
| Derby County | 10 | 0 | 4 | 6 | 8 | 29 |
| Doncaster Rovers | 10 | 4 | 2 | 4 | 18 | 14 |
| Fulham | 10 | 2 | 2 | 6 | 8 | 24 |
| Gateshead AFC | 1 | 1 | 0 | 0 | 2 | 1 |
| Glossop North End | 26 | 7 | 7 | 12 | 31 | 44 |
| Grantham Town | 1 | 1 | 0 | 0 | 4 | 1 |
| Grimsby Town | 26 | 10 | 6 | 10 | 41 | 41 |
| Harrogate Town | 1 | 0 | 0 | 1 | 0 | 1 |
| Hartlepool United | 1 | 0 | 0 | 1 | 0 | 6 |
| Hednesford Town | 1 | 0 | 1 | 0 | 4 | 4 |
| Huddersfield Town | 4 | 2 | 1 | 1 | 11 | 5 |
| Hull City | 14 | 4 | 3 | 7 | 18 | 27 |
| Leeds City | 15 | 6 | 4 | 5 | 17 | 19 |
| Leicester City | 22 | 6 | 5 | 11 | 30 | 37 |
| Leicester Fosse | 14 | 3 | 2 | 9 | 10 | 23 |
| Leyton Orient | 14 | 4 | 2 | 8 | 13 | 20 |
| Lincoln City | 34 | 10 | 8 | 16 | 47 | 54 |
| Liverpool | 3 | 0 | 0 | 3 | 4 | 11 |
| Long Eaton Rangers | 1 | 1 | 0 | 0 | 1 | 0 |
| Loughborough | 8 | 6 | 1 | 1 | 20 | 4 |
| Luton Town | 9 | 0 | 3 | 6 | 11 | 24 |
| Manchester City | 10 | 2 | 1 | 7 | 8 | 31 |
| Manchester United | 14 | 0 | 5 | 9 | 9 | 24 |
| Mansfield Town | 2 | 1 | 0 | 1 | 4 | 5 |
| Middlesbrough | 7 | 1 | 2 | 4 | 11 | 19 |
| Netherfield | 2 | 1 | 1 | 0 | 4 | 1 |
| New Brighton Tower | 6 | 2 | 1 | 3 | 12 | 14 |
| Newcastle United | 4 | 2 | 0 | 2 | 7 | 9 |
| Newcastle West End | 1 | 1 | 0 | 0 | 6 | 2 |
| Newport County | 1 | 0 | 0 | 1 | 1 | 2 |
| Newton Heath | 6 | 2 | 0 | 4 | 5 | 12 |
| Nottingham Forest | 5 | 0 | 0 | 5 | 4 | 14 |
| Notts County | 2 | 1 | 0 | 1 | 3 | 4 |
| Oldham Athletic | 6 | 0 | 1 | 5 | 3 | 15 |
| Plymouth Argyle | 1 | 0 | 0 | 1 | 0 | 3 |
| Port Vale | 20 | 7 | 5 | 8 | 27 | 26 |
| Preston North End | 6 | 2 | 1 | 3 | 4 | 7 |
| Scunthorpe United | 1 | 1 | 0 | 0 | 1 | 0 |
| Sheffield United | 3 | 1 | 0 | 2 | 5 | 4 |
| Sheffield Wednesday | 2 | 0 | 0 | 2 | 1 | 7 |
| Shrewsbury Town | 1 | 0 | 0 | 1 | 0 | 1 |
| Slough Town | 1 | 0 | 0 | 1 | 0 | 6 |
| Small Heath | 12 | 1 | 3 | 8 | 12 | 37 |
| South Bank | 1 | 1 | 0 | 0 | 4 | 0 |
| South Shields AFC | 1 | 1 | 0 | 0 | 1 | 0 |
| Southend United | 2 | 0 | 1 | 1 | 1 | 2 |
| Southport | 1 | 0 | 0 | 1 | 0 | 4 |
| Stockport County | 22 | 10 | 8 | 4 | 30 | 21 |
| Stockton | 1 | 1 | 0 | 0 | 6 | 0 |
| Stoke | 4 | 1 | 2 | 1 | 5 | 8 |
| Stoke City | 1 | 0 | 0 | 1 | 1 | 3 |
| Sunderland | 2 | 1 | 1 | 0 | 4 | 1 |
| Tottenham Hotspur | 2 | 0 | 1 | 1 | 1 | 3 |
| Walsall | 11 | 3 | 4 | 4 | 14 | 19 |
| Watford | 2 | 1 | 0 | 1 | 3 | 5 |
| West Bromwich Albion | 16 | 5 | 2 | 9 | 21 | 41 |
| West Ham United | 1 | 0 | 0 | 1 | 1 | 2 |
| Witton Albion | 2 | 1 | 0 | 1 | 2 | 2 |
| Wolverhampton Wanderers | 12 | 3 | 2 | 7 | 5 | 14 |
| Woolwich Arsenal | 16 | 3 | 2 | 11 | 14 | 44 |
| Yeovil and Petters United | 1 | 0 | 0 | 1 | 1 | 2 |