Lazio v Viktoria Plzen, 13 March 2025
| Score | 1-1 |
| Referee | Danny Makkelie |
| Competition | UEFA Europa League Round of 16 2nd leg |
| Venue | Stadio Olimpico, Rome |
| Attendance | 39,547 |
Kick-off: 6.45pm local
.
Lazio won 3-2 on aggregate
1 Lazio
Manager: Marco Baroni
1 Viktoria Plzen
Manager: Miroslav Koubek