Fiorentina v Polissya Zhytomyr, 28 August 2025
| Score | 3-2 to Fiorentina |
| Competition | UEFA Conference League Qualifying play-off 2nd leg |
| Attendance | 5,033 |
Played at the Mapei Stadium – Città del Tricolore in Reggio Emilia due to renovation works at the Stadio Artemio Franchi in Florence.
.
Fiorentina won 6-2 on aggregate