| Score | 3-2 to Barnsley |
| Referee | Stephen Martin |
| Competition | League One |
| Venue | Weston Homes Community Stadium, Colchester |
| Attendance | 3,265 |
View Colchester United v Barnsley head to head
| George Moncur | 66 |
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| Chris Porter | 70 |
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| Chris Porter for Joe Edwards | 46 |
| Kane Vincent-Young for Matthew Briggs | 66 |
| Macauley Bonne for Gavin Massey | 74 |
| Conor Hourihane | 18 |
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| Adam Hammill | 39 |
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| Ivan Toney | 60 |
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| Ben Pearson | 90 | Y |